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KMID : 0881720180330060516
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Journal of Food Hygiene and Safety
2018 Volume.33 No. 6 p.516 ~ p.520
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Characterization of the Bacteriocin from Enterococcus faecium CJNU 2008
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Seo Souk-Jin
Yang Jung-Mo
Moon Gi-Seong
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Abstract
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Bacteriocin is a proteinaceous compound produced by microorganisms showing antimicrobial activities. In this study, the physicochemical properties of the bacteriocin produced by Enterococcus faecium CJNU 2008 strain were characterized. Partially purified bacteriocin showed stabilities against heat treatments at 100℃ for 30 min and 121℃ for 15 min and against solvents treatments such as methanol, ethanol, acetone, acetonitrile and chloroform. The bacteriocin also exhibited stabilities against lipase and α-amylase treatments but the stability was abolished at protease treatment, indicating that the antimicrobial agent from E. faecium CJNU 2008 was a proteinaceous bacteriocin. The bacteriocin also showed bactericidal mode of action against Listeria monocytogenes. The Molecular mass of the bacteriocin was estimated to be under 6.5 kDa by a tricine-SDS-PAGE analysis. The bacteriocin was purified by HPLC. Further studies toward biochemical analysis of the bacteriocin are needed in near future.
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KEYWORD
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Bacteriocin, Enterococcus faecium, Listeria monocytogenes, Bactericidal activity, Purification
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